Chromatin immunoprecipitation and deep sequencing in Xenopus tropicalis and Xenopus laevis

Analysis of Developmental Epistasis by Chromatin Immunoprecipitation in Xenopus laevis

Proposal for sequencing the Xenopus tropicalis genome

Preface to Xenopus White Paper. This paper is being submitted after the recent announcement that the Joint Genome Institute is planning an extensive effort to sequence the Xenopus tropicalis genome. As discussed below, the current plan from JGI is for 6X coverage of the genome using a shotgun sequencing strategy. This paper is being submitted because in addition to the JGI commitment, it would ...

Comparison of induction during development between Xenopus tropicalis and Xenopus laevis.

Several in vitro systems exist for the induction of animal caps using growth factors such as activin. In this paper, we compared the competence of activin-treated animal cap cells dissected from the late blastulae of Xenopus tropicalis and Xenopus laevis. The resultant tissue explants from both species differentiated into mesodermal and endodermal tissues in a dose-dependent manner. In addition...

A Xenopus tropicalis oligonucleotide microarray works across species using RNA from Xenopus laevis

Microarrays have great potential for the study of developmental biology. As a model system Xenopus is well suited for making the most of this potential. However, Xenopus laevis has undergone a genome wide duplication meaning that most genes are represented by two paralogues. This causes a number of problems. Most importantly the presence of duplicated genes mean that a X. laevis microarray will...

Effects of freezing and activation on membrane quality and DNA damage in Xenopus tropicalis and Xenopus laevis spermatozoa.

There is growing concern over the effect of sperm cryopreservation on DNA integrity and the subsequent development of offspring generated from this cryopreserved material. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure t...